API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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Materials Required but not provided The following materials may be required but are not provided: Well 8 for 12B and 20 for 24E is not overlayed with oil for oxidase- positive, miscellaneous Gram-negative bacilli.
Well 8 Indole production – add 2 drops of Indole Kovacs reagent.
The expected results is positive. Sodium malonate is the sole carbon source and this inhibits the conversion of succinic acid to fumaric acid. Refer to the procedure chart for a condensed version of the following procedures.
Before use, perform an oxidase test on the organism to be galerue. This is a negative reaction.
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Incubation Reseal the inoculated rows with the adhesive seal and write the specimen identification number on the end tag with a marker pen. The percentage figure shown against the organism name is the percentage share of the probability for that organism as a part of the total probabilities for all choices. H 2 S reacts with ferric salts in the medium to form a black precipitate.
Miscellaneous Gram-negative bacilli – Weakly positive reactions are recorded as negative results.
General Aspects and Perspectives. Yellow-green is indicative of a negative result. Well 1 lysine Well 2 ornithine Well 3 H 2 S. Each strip consists of 12 different biochemical substrates. Twelve substrates provide insufficient data to speciate within this group as a single aberrant reaction may result in an incorrect identification. Using galegie sterile Pasteur pipette, transfer one drop of the peptone water culture into the appropriate volume see Procedure Chart of sterile saline solution 0.
In 200e, motility and nitrate test should be performed for miscellaneous Gram-negative bacilli. Materials Required but not provided The following materials may be required but are not provided:. Using the results obtained, the indices of the positive reactions are circled. Utilisation of Na malonate at the same time that ammonium sulphate is utilised galerid the nitrogen source produces sodium hydroxide resulting in increased alkalinity and a blue colouration.
Organism identification is based on pH change and substrate utilisations as established by published reference methodologies 4,6,8,9. Prolonged incubation, insufficient incubation, improper filling of wells, or inadequate inoculum may lead to false results. Indole Kovacs reagent gwlerie a pink-red complex with indole.
All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites and give a positive reaction. The results should be interpreted as follows:.
Hydrolysis of gelatin is indicated by dispersal of the black particles throughout the well. Please click on a thumbnail to view the full size image and open the gallery. The following chart gives the expected results on the Microbact System after an hour incubation: Strips stored in this manner must be used within 7 days.
Reactions obtained using the Microbact System may differ from published results using other substrate formulations. Identification of Bacteria by Computer: Argine dihydrolase converts arginine into ornithine, ammonia and carbon dioxide.
The 12A 12E strip should be read at hours. Bromothymol blue is the indicator. Green or blue is positive reaction. These strips are intended for in vitro use only; for use by qualified laboratory personnel using aseptic techniques and established precautions against microbiological hazards. The lysine and ornithine decarboxylase reactions should be carefully interpreted. The results of tests for oxidase, nitrate reduction and motility are included as part of the reaction pattern.
Inoculation The wells of individual substrate sets can be exposed by cutting the end tag of the sealing strip and slowly peeling it back. Using the results obtained, from each group of three reactions a 9 nine digit code number is produced. H 2 S is produced from thiosulphate.
One drop of Nitrate reagent A and 1 drop of Nitrate reagent B is added to the well. Quality control The overall performance of the system should be monitored by testing appropriate control strains.
Acetoin is produced from glucose indicated by the formation of a pink-red complex after the addition of alpha-naphthol and creatine. The sum of these indices in each group of three reactions forms the code number.